GRE-ALL Coleoptera

November 11, 2025

Sample information
Picture
Photos by: A'yanni L
Location No map location data available.
Collection date 08/26/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time of collection: Around 3pm​

Location of collection: In between the science center and library near the parking area on Library Dr​

Environment description: grassy/near shrubs and underneath the area of a tree​

Method of collection: sweep net
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Other
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Testing was used and I’m confident that the results are accurate
Wolbachia 16S sequence
TTATGAGCAGGATAGTAGGGACTTCCTTAAGAATTTTAATTCGATTAGAA TTAGGAACTACtatagCTTTAATTGGAAACGATCAGATTTATAATGTAAT TGTAACAGCGCATGCTTTTATTATAATTTTTTTCATAGTAATACCAATTA TAATTGGAGGGTTTGGAAATTGATTAGTCCCTTTAATAATTGGTGCTCCC GATATAGCATTTCCTCGTTTAAATAATATAAGATTTTGACTTTTACCTCC TTCTTTGACTTTCTTAATTTTAAGAAGCCTAGTAGAAAGAGGAGCAGGAA CTGGATGAACAGTTTATCCTCCTTTATCCTCAAATTTAGCTCACGGAGGA TCTTCTGTAGATATAGCAATTTTTAGACTACACTTAGCGGGAATTTCTTC TATTTTAGGTGCTGTAAATTTTATTTCAACTATTATTAATATACGAACAT ACGGAATAACTTTTGAAAAAATACCTTTATTTGTATGATCAGTATTAATT ACTGCTGTACTCTTATTATTATCCTTACCTGTATTAGCTGGAGCAATTAC TATACTTTTAACAGATCGAAATATTAATACCTCTTTCTTTGACCCAGCAG GAGGGGGAGACCCTATTTTATACCAACACTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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