DEM-DYP-Diptera

November 12, 2025

Sample information
Picture
Photos by: Diana Patel
Location
Collection date 08/25/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
•Date: Aug 25, 2025
•Time: 1:20 PM
•Location: Outside GSU Science Center
•Habitat: grassy area with leaf litter & shrubs
•Method: manual capture-Sweep net
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit.
DNA extraction location
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

I chose a medium confidence level because the COI control band appeared clearly, showing that my DNA extraction and PCR worked. The Wolbachia 16S band was either faint or not visible, so it is uncertain if Wolbachia DNA was present or just below detection level. Since the positive and negative controls both worked as expected, I can trust that my results are valid, but I cannot fully confirm infection without sequencing.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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