MAN-VMP Hemiptera

November 12, 2025

Sample information
Picture
Photos by: Victoria P.
Location
Collection date 08/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Collected in short grass behind the greenhouse near the pond on campus
  • Used gloved hands and a specimen jar to collect
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My well only contained a band around the 700 bp line, and Insect CO1 PCR product ends around the 708 bp line.
Wolbachia 16S sequence
Arthropod COI sequence
GAGCAGGTATAGTAGGATCCGCTATAAGACTAATTATCCGAATCGAACTA GGACAACCaggtagATTCATTGGGGATGATCAAATTTATAACGTAATCGT AACAGCTCACGCCTTCGTAATAATTTTCTTCATAGTAATACCTATTATAA TTGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGCTCCCGAC ATAGCATTCCCTCGTATAAATAACATAAGATTTTGATTATTACCTCCTTC ATTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCTGGAACCG GATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCTCACAGAGGAGCA TCCGTAGACTTAGCAATTTTTTCACTCCACTTAGCCGGGGTATCTTCAAT CTTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGACCCGCAG GAATAATCCCTGAACGAATTCCTTTATTTGTATGATCGGTAGGGATTACT GCCTTACTTCTACTACTATCATTACCTGTGCTAGCAGGTGCTATTACAAT ATTATTAACTGATCGAAATTTTAACACATCATTTTTTGATCCCTCAGGTG GAGGAGACCCTATTTTATACCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hemiptera was found to be negative for Wolbachia.
Report Inappropriate Post