Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/17/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | abandon garden, very grass area, fenced off |
| Putative identification | Arthropoda Insecta Coleoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | PCR results were clear |
| Wolbachia 16S sequence |
GTGGGAACATCCCTAAGAATACTAATTCGTACAGAATTAGGAAACCCttt atCTCTAATTGGAGACGACCAAATTTATAACGTAATTGTAACAGCACATG CTTTTATTATAATTTTCTTTATAGTTATACCAATAATAATCGGGGGATTC GGAAATTGATTAGTCCCCCTCATACTAGGAGCACCTGACATAGCATTCCC ACGATTAAATAATATAAGATTCTGGCTTCTTCCACCATCATTAACTCTTC TTCTAATAAGAAGAATCATTGACAAAGGTGCAGGGACAGGTTGAACTGTT TACCCACCCTTATCAGCTAATGTGGCACATGAAGGACCTTCTGTAGACTT GGCTATTTTCAGACTCCATATAGCTGGTATTTCCTCAATTTTAGGGGCCA TTAATTTTATCTCTACAGTTATAAATATGCGACCTAAGGGTATAACCCTT GACCGTATACCACTTTTTGTATGAGCAGTTAAAATTACTGCTATTCTCCT TCTTCTATCCTTACCTGTATTAGCAGGAGCTATCACTATGCTACTAACAG ACCGTAATATCAACACTTCCTTCTTTGACCCCGCAGGAGGAGGTGATCCT ATCTTATACCAACACTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid