MAN-SMM Orthoptera

November 12, 2025

Sample information
Picture
Photos by: Stanton M
Location
Collection date 08/27/2025
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected in long grass by the aquaponics center

Used a swoop net and a jar
Putative identification Arthropoda

Methods
Extraction kit
DNA extraction location head and lower body
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

A DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I felt pretty confident going in, given that the insect order Orthoptera had one of the highest infection rates, and most gel readings were positive
Wolbachia 16S sequence
AGACGTATTCACCGTGACATGCTGATCCACGATTACTAGCGATTCCAACT TcatcgttctcGAGTTGCAGAGTACAATCCGAACTGAGATGTCTTTTGGG GATTAGCTTAGGCTTGCGCACCTTGCAACCCATTGTAGACACCATTGTAG CACGTGTGTAGCCCACTCCATAAAGGCCATGATGACTTGACATCATCCCC ACCTTACTCCAGCTTATCACTGGCAGTTTCCTTAAAGTGCTCAGCATTAC CTGATGGCAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAA CATCTCACGACACGAGCTGACGACAGCCATGCAACACTTGTGTAAAATCC gacCGAACCGACCCTATCCCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
GAGCTTGATCTGCTATAGTAGGAACTTCTTTAAGAATTTTAATTCGAACA GAATTAGGTCAACccgtatcattaATTGGAGATGATCAAATTTATAACGT AATTGTTACTGCTCACGCTTTTGTAATAATTTTTTTTATAGTAATACCAA TTATAATTGGTGGATTTGGAAATTGATTAGTTCCTTTAATATTAAGAGCT CCTGATATAGCTTTCCCTCGAATAAATAATATAAGATTTTGATTATTACC ACCTTCTTTAACTCTCCTATTATCCAGAAGATTAGTAGAAAACGGTGCAG GAACCGGATGAACAGTGTACCCTCCACTATCATCTCTAATTGCCCACAGA GGTGCATCCGTTGATCTTGCAATTTTTTCTCTTCATTTAGCTGGAATTTC ATCAATTTTAGGAGCAATTAATTTTATTTCTACTATAATTAATATACGAG CTCCAGGTATAACTTTAGATCAAACTCCTTTATTTGTATGAGCTGTAGGT ATTACAGCCCTTCTTCTTCTTTTATCTCTTCCAGTTCTAGCAGGAGCTAT TACTATACTTTTAACAGATCGTAATTTAAATACTTCTTTTTTTGATCCTG CTGGAGGAGGAgatCCTATTTTATATCAACACTTATTTTGATTTTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arthropoda was found to be postive for Wolbachia.
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