Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/25/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | NCSSM |
| Observations | |
| Putative identification | Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Green |
| Gel images |
|
| Protocol notes | I dissected the lady beetle, removing its wings and exoskeleton, and then its abdomen. I crushed the abdomen with a pestle, and that is what I ended up using for the amplification of the DNA. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | Our arthropod negative control ended up showing up as positive, which could mean that there was unintentional cross-contamination during the procedure. While trying to keep that from happening to the best of our abilities, there is always going to be some possibility of unintentional contamination. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download AB1
|
| Summary | The Harmonia axyridis was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid