Multicolored Asian Lady Beetle (Harmonia axyridis) – Sample Two

December 4, 2025

Sample information
Picture
Photos by: Alina Y.
Location
Collection date 09/25/2025
Captive / Cultivated? Wild-caught
Group NCSSM
Observations

Our sample was captured outside the Residence Hall of NCSSM-Morganton. It was found in the shrubs between the Residence Hall and Jeter.
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain Cyber Green
Gel images
Protocol notes

Standard protocol for processing the whole arthropod was followed—no additional notes to report.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The Multicolored Asian Lady Beetle has been found to have Wolbachia in other regions of the world, but no such instance has been found in Burke County, North Carolina, yet.
Wolbachia 16S sequence
Arthropod COI sequence
GGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAAT TGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATA CCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTC CACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGT AGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCT TCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCA TTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGT TCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACT GACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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