Acrididae

December 4, 2025

Sample information
Picture
Photos by: Ayesha S
Location
Collection date 09/02/2025
Captive / Cultivated? Wild-caught
Group Benedictine University
Observations

Orthoptera found in grassy area by the campus quad
Putative identification Arthropoda Insecta Orthoptera Acrididae Melanoplus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence levels are high because my BLAST results were almost 100%. All the controls worked an di was given acceptable and clean data to analyze.
Wolbachia 16S sequence Download FASTA    Download AB1
>w15R.ab1 (617 bp) ATTTAAATTTCGATCAGTTAATAGTATAGTGATTGCTCCTGCTAAGACAGGTAAAGATAATAAAAGTAAAATTGCTGTAA TTAAAACAGATCATGTAAATAAAGGTAAAAAATTTAATGAATGGGTTTTAATATGTATATTTAAAATTGTTACAATAAAA TTAATAGCTCCTATAATGGATGAGATTCCAGCAATATGAAGAGAAAAAATTGATAAATCAACAGAAGGTGAATTATGTCC AATATTTGAAGATAAAGGTGGGTATAAAGTTCATCCTGTTCCTACTCCTATTTTTATTATGTTTCTTATAATAAGCAAAA ATAATGAGGGTGGTAAAAGTCAAAATCTTATATTATTTATTCGTGGGAAAGCTATATCAGGGGCTCCAAGTATTATAGGA ATTAATCAATTTCCAAAACCTCCAATTATAAATGGTATAACTATAAAAAAAATTATTACTAAAGCATGGGCTGTAATAAT TGAATTATAAAATTGATCATTATTAATAATTGAAGATGGTGTTCCTAATTCTAATCGAATTATTTTTTTTTTTTATGGGT GGGGGGGGTCCAGCTCAGAGTCCAAACCAAATATTATATTGTTCTGTTCACAAATAT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
>4-CA_B4_CoR.ab1 (616 bp) GAnnTAnTTAAATTTCnATCAGTTAATAATATTGTAATAnCTCCTGCTAATACAGGAAGTGATAATAATAAAAGGAGTGC TGTAATAGCTACTGATCAAACAAATAATGGTGTTTGGTCTAGTGTTATTCTTTCTGATCGTATATTAATTGCTGTTGTAA TAAAATTGACTGCCCCTAGAATTGATGAAACACCAGCTAAGTGAAGAGAGAAAATGGCTAAATCAACTGACGCTCCACCA TGTGCAATTGCTCCAGCGAGTGGGGGGTAAACTGTTCATCCTGTACCAGCTCCATTATCAACCATAGATGAGGTAAGTAA AAGGGTTAGTGATGGTGGTAAAAGTCAAAAACTTATATTATTTATTCGTGGAAAAGCTATATCTGGTGCTCCAATTATTA ATGGTACAAGTCAATTACCAAATCCTCCAATTATAATAGGTATTACTATAAAGAAAATTATTACAAATGCGTGGGCTGTA ATAATTACATTATAGATTTGGTCATCTCCAATTAnAGATCCTGGTTGTCCTAGTTCTGCTCGAATAATTATTCTTATAGA AGTTCCTACTATTCCAGCTCATGCTCCAAATATAAAGTATAAGGTACCAATATCTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Melanoplus was found to be negative for Wolbachia.
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