Ladybug

January 30, 2026

Sample information
Picture
Entry by: Andrew K.
Location
Collection date 08/31/2031
Captive / Cultivated? Wild-caught
Group Hamburg Area High School
Observations

Red with black spots, Wings under a hard shell, ~2-3mm diameter (circular/oval-shaped shell)
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Harmonia Harmonia axyridis

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBG
DNA stain SYBR-SAFE
Gel images
Protocol notes

Standard.

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

While the lab itself did work, and everything went smoothly. Due to the blurriness of the lines in our gel and its images, it is hard to tell whether the Wolbachia node should or should not be present. This could also have occurred during the process of creating the gel.
Wolbachia 16S sequence
Arthropod COI sequence Download AB1
TTATGGGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAA GATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCT ATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAA CATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGAT GAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTA GCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGA TAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAA TTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAA CATTTATTTTGATTTTTTGC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Harmonia axyridis was found to be negative for Wolbachia.
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