Sample information
Picture	Photos by: Dr. Replogle
Location
Collection date	05/20/2021
Captive / Cultivated?	Wild-caught
Group	The Summit Country Day School
Observations
Putative identification	Arthropoda Hexapoda Insecta Hemiptera Cicadidae Magicicada Magicicada septendecim
Methods
Extraction kit	Thermoscientific GeneJet Genomic Purification
DNA extraction location	Partial abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Magicicada septendecim was found to be negative for Wolbachia.