Japanese Burrowing Cricket, State College, PA – Wolbachia Results

March 12, 2026

Sample information
Picture
Entry by: Emily Amato
Location
Collection date 09/20/2025
Captive / Cultivated? Wild-caught
Group Penn State University
Observations

The Japanese Burrowing Cricket was caught near a tree in its natural habitat.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer 1X TAE
DNA stain Ethidium Bromide
Gel images
Protocol notes

DNA Extraction: The arthropod was crushed well, and the abdomen was efficiently dissected. No exoskeleton was noticed to be included.

Analysis: My controls and sample worked as expected.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

All of my controls worked as expected. I had no problems with the protocol, and I was not surprised by any bands on the gel. Therefore, I am confident my arthropod is infected with Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be postive for Wolbachia.
Report Inappropriate Post