Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 12/12/2025 |
| Captive / Cultivated? | Wild-caught |
| Group | Coalinga High School |
| Observations | the weather was chilly when bee was found, the bee itself was very isolated, the be was found alive than was frozen for death, it was found in the fields on the ground with rocks. |
| Putative identification | Arthropoda Insecta Hymenoptera Anthophila |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniOne |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | followed mini one protocol |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We as a group are very confident about our results, we clearly went wrong in a certain step that clearly affected our results to have a positive Wolbachia gel or any results as a matter. That being said it also affect our result in our Arthropod. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly