Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Hawaii Community College |
| Observations |
|
| Putative identification | |
Methods |
|
| Extraction kit | Edwards Buffer |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | 1X TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level |
Lanes 2 and 3 are CO1 specific PCR from two different Glaber ants. Lanes 4 and 5 are Wolbachia specific PCR. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Parson Spider
Wasp
Caterpillar
Fruit Fly
Fly