bombus

April 8, 2026

Sample information
Picture
Entry by: Tyler P
Location
Collection date 09/11/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

it has wings with black and yellow fur with a stinger. also had black legs and arms.
Putative identification Arthropoda Insecta Hymenoptera Apidae Bombus

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

extract dna from bombus

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

very confident, went through the procedure and gave the right results
Wolbachia 16S sequence
Arthropod COI sequence
GNNNNGNNAATTCGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACATCCAGGAATATGAATTAATAATGATC AAATTTATAATTCATTAGTTACTAGACATGCATTTTTAATAATTTTTTTTATAGTTATACCATTTATAATTGGAGGATTT GGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCACGAATAAATAATATTAGATTTTGATTATT ACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGGAACAGGTTGAACCGTATATCCCCCTT TATCATCTTATTTATTTCATTCATCACCTTCAGTAGATATTGCAATTTTTTCTTTACATNTAACAGGAATTTCTTCTATT ATTGGTTCATTAAATTTTATNGNTNCNATTATATTAATAAAAAATTTTTCATTAAATTATGATCAAATTACTTTATTTTC ATGATCTGTATGTATTACAGTNTTATTATTAATTTTATCATTACCAGTTTTAGCNGGAGCAATTACTATACTTCTTTTTG ATCGAAATTTTAATACATCATTTTTTGATCCAATAGGAGGTGGAGATCCAATTTTATATCAACATTTATTTTGANTTTNN NNNCN
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Bombus was found to be negative for Wolbachia.
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