Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 02/09/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Used a dip net to collect our insect. |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | head and lower body |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | A DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | My gel results were inaccurate. We also had a mix-up mid-way through the entire project, where our instructor threw us off, and we had to start a whole new sample mid-way. This event kind of threw me off and lowered my confidence in my sample. Besides that Id say everything else was pretty good, and the other labs all went smoothly, and I believe I was very accurate with my work. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
MAN-JAB
MAN-MRC Hymenoptera
Hempitera DEM-TMJ
DEM-CML Hymenoptera
man-zzr Dermaptera