MAN-IGE Coleoptera

April 14, 2026

Sample information
Picture
Entry by: Isabella E.
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Time of Collection: 3:25 p.m.​

​
Location of Collection: Savannah, GA on the Georgia Southern Armstrong Campus​

  • Location 1: 31°58’48″N 81°09’38″W, arbory on side of college of science and mathematics building ​
  • Location 2: 31°58’50″N 81°09’34″W, Fields by greenhouse​
  • Location 3: 31°58’48″N 81°09’43″W, fountain in between The Learning commons, lane library, and Hawes Hall​

​
Environments of Collection: dirt, wet dirt near fountain, abrory/plants, and ant hills​

​
Methods of Collection: Digging, tweezers, collection tray, looking on spider plant leaves​
Putative identification Arthropoda Insecta

Methods
Extraction kit pcr
DNA extraction location Lab
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Gel results confirm presence of Wolbachia. Line is faint, but clearly present.
Wolbachia 16S sequence
Arthropod COI sequence
GGCAGGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAG GAACTagattaagATTAATTGGAAACGACCAAATTTATAATATAATTGTT ACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAAT TGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATA TAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCT TTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGG ATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTT CAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATT TTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGG TATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAG CAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATA CTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGG GGGAGACCCAATTTTATACCAACATTTATTTTGATTTTTTGGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be postive for Wolbachia.
Report Inappropriate Post