MANS-EEC Blattodea

April 14, 2026

Sample information
Picture
Entry by: Emma C
Location
Collection date 01/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

No wings

6 true legs

Lacks prolegs

Has mandible

All legs look similar

Abdomen has finger-like appendages

Lacks pinchers, has tail-like attachments

2 abdominal appendages that are shorter than legs

Abdominal appendages not fork-like
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The order of my insect has a high statistic for having Wolbachia present, the gel showed a clear second bar in the Wolbachia gene column
Wolbachia 16S sequence
Arthropod COI sequence
TGGATAGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCA AccttgttcATTAATTGGAGATGATCAAATCTATAATGTTATTGTAActg CTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGA GGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGC ATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTAT CTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGA ACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGT AGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAG GTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATA AGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATT ATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTAT TAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGT GATCCTATTTTATATCAACATTTATTTTGATTTTTTGGTCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be postive for Wolbachia.
Report Inappropriate Post