CHI-MME Blattodea

April 15, 2026

Sample information
Picture
Entry by: Madeleine E
Location
Collection date 01/14/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

-The insect had a preexisting fungal infection located on its back abdomen.
Putative identification Arthropoda Insecta Blattodea

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

I am confident that this insect was infected with something, I am not 100% sure about accidental contamination or if the preexisting infection affected testing.
Wolbachia 16S sequence
CATACCTATTCGAAGGGATAGGGTCGGTTCGGCCGGATTTCACACAGGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGTAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGATTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATAC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
TTTATTTTATCTTCGGAGCTTGATCTGGAATGGTAGGGACATCTTTAAGAATATTAATCCGAGCAGAACTAAATCAACCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTCACGCTTTTGTTATAATTTTCTTTATAGTTATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATTTCCTCGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTTTATCTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGCTGGGACTGGTTGAACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGATTTAGCTATTTTCTCATTACATCTTGCAGGTGTATCATCAATTTTAGGTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAATATAAGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATTATTATTATTATCCTTACCAGTTCTTGCAGGTGCAATTACAATATTATTAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCAGGGGGAGGTGATCCTATTTTATATCAACATTTATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattodea was found to be postive for Wolbachia.
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