CHI-GG Orthoptera

April 15, 2026

Sample information
Picture
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Georgia Southern University, Arts Dr.
  • Collected with hand and jar
  • Near long grass that can help the insect camouflage
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The process was done correctly with no complications.
Wolbachia 16S sequence
Arthropod COI sequence
TAGTAGGAACATCAATAAGAATAATTAttncgtGCTGAATTAGGACAAcc attatCTCTAATTGGAGATGATCAAATCTATAATGTTATCATCACAGCAC ATGCATTCGTAATAATTTTCTTTATAGTTATACCAATTATAATTGGTGGA TTTGGTAATTGACTAGTACCATTAATAATCGGAGCACCAGATATAGCATT CCCACGAATAAATAACATAAGATTTTGATTATTACCACCATCATTAACCT TATTACTCACGTCCTCTATAGTTGATATAGGAGCCGGAACTGGTTGAACA GTTTACCCTCCGCTTGCAGGAGCAATTGCACATGGTGGTGCTTCAGTAGA CCTAGCCATTTTTTCATTACACTTAGCCGGAGTTTCATCAATTTTAGGTG CAGTAAACTTCATCACAACAGTAATTAATATACGATCAGAAAGAATAACA TTAGATCAAACACCCTTATTTGTTTGATCAGTAGCTATTACAGCATTACT TCTATTATTATCATTACCAGTTCTTGCAGGAGCAATTACAATATTATTAA CAGATCGAAATCTAAATACATCATTCTTTGACCCTGCAGGTGGTGGAGAT CCAATTCTATATCAACATTTATTCTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
Report Inappropriate Post