DEM-MGC Coleoptera

April 15, 2026

Sample information
Picture
Entry by: GracieC
Location
Collection date 01/28/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught by digging in the dirt and using a jar to collect it.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My specimen does not have Wolbachia. I am confident in this outcome due to the careful precision I used when working on my labs in class to receive this result.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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