Arthropod 6: P #3

June 15, 2026

Sample information
Picture
Entry by: Aubrey S, Sarah W, Brooke T, Rebecca S.
Location
Collection date 05/13/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

(Location of collection)
Putative identification Arthropoda Insecta Coleoptera Coccinellidae Coccinella Coccinella septempunctata

Methods
Extraction kit Qiagen DNeasy Blood and Tissue Kit
DNA extraction location Gonads dissected
Single or Duplex PCR Single Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer TBE
DNA stain SeeGreen
Gel images
Protocol notes

(Dissection)

(Grinding DNA with 180 uL of ATL Buffer for PCR)

(Separated DNA after PCR)

(Loading Gels)

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

If our arthropods had wolbachia, there would be a bright band aligning with the +DNA control (the brightest band). Because the arthropods DNA does not align, we know they do not have wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coccinella septempunctata was found to be negative for Wolbachia.
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