Ladybug #1

June 3, 2026

Sample information
Picture
Entry by: Anna S
Location
Collection date 05/13/2026
Captive / Cultivated? Wild-caught
Group Winston Knoll Collegiate
Observations

I found this ladybug in the grass field behind Winston Knoll Collegiate. I collected this ladybug at 11:14am, it was sunny out with a temperature of 25 degrees celsius. It was caught in the spring season on May 13th, 2026.
Putative identification Arthropoda Insecta Coleoptera Coccinellidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer 1X TBE
DNA stain SeeGreen
Gel images
Protocol notes

DNA extraction: I made sure the ladybug was thoroughly crushed. I dissected the abdomen out carefully, and did not include the exoskeleton.

Gel electrophoresis lanes for CO1 arthropod PCR:

1- NEB NO551L ladder

2- Ladybug #1

3- Big Black Beetle #2

4-Ladybug #3

5- Small Black Beetle #4

6- +arthropod control

7- -Arthropod control

8- +DNA control

9- Water

Results
Wolbachia presence
Confidence level
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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