Sample information
Picture	Entry by: Bhargavi Shinde
Location
Collection date	04/20/2026
Captive / Cultivated?	Wild-caught
Group	Thomas Jefferson High School for Science and Technology
Observations
Putative identification	Arthropoda
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit.
DNA extraction location	Partial abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Arthropoda was found to be negative for Wolbachia.