Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | |
| Putative identification | Arthropoda Arachnida Araneae Agelenidae Eratigena Eratigena agrestis |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | Because the sample was larger, we used a column kit for DNA extraction. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GGGGCTTGGTCTGCTATAGTTGGAACAGCTATAAGAGTTCTTATTCGAATTGAGTTAGGTCAACCTGGTAGATTTTTGGGTGATGATCATTTATATAATGTTATTGTTACTGCTCATGCTTTTATTATAATTTTTTTTATAGTTATACCAATCATGATTGGTGGGTTTGGTAATTGATTAGTTCCTTTAATATTGGGAGCTCCAGATATAGCTTTTCCTCGTATAAATAATTTAAGTTTTTGATTGTTACCGCCTTCTTTGATTATATTGTTTATTTCTTCTATAGTTGAGATAGGTGTTGGGGCGGGGTGAACTATTTATCCTCCTTTAGCTTCTTCTATTGGTCATTTTGGTAGATCTGTTGATTTTGCTATTTTTTCTTTGCATTTGGCTGGGGCTTCTTCTATTATAGGTGCTATTAATTTTATTTCTACTATTTTTAATATACGATCTGTTAGAATAACTATAGAGAAGGTTCCTTTATTTGTATGATCTGTTTTAATTACTGCTGTTTTGTTATTATTGTCTTTACCTGTATTAGCTGGTGCTATTACTATATTATTAACTGATCGAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Eratigena agrestis was found to be negative for Wolbachia. |
Cicurina (Meshweaver spider)
Tufted Clusterfly
Juvenile Pillbug
Dark-winged Fungus Gnat
Elm Seed Beetle