Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | College of Eastern Idaho |
| Observations | |
| Putative identification | Arthropoda Arachnida Opiliones |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit. |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | 1X Agarose Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SeeGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
CCATTAGGCTACNTANNCTCGAATTGAATTGGGACAACCTGGATCATTCCTAAATGATAGATCAANTTTATAACGCTATTGTAACTGCTCATGCCTTTGTTATAATTTTCTTTCTAGTAATACCCATTTTAATTGGAGGATTCGGAAACTGATTACTCCCTTTAATACTAGGAGCCCCTGACATAGCCTTCCCCCGACTTAACAACATAAGATTCTGACTCCTCCCCCCTTCTTTTTTTCTTTTACTATCCTCTGCTGTGGTGGAAAACGGAGCGGGAACTGGATGAACTGTTTACCCCCCCCTATCAAGAAATATTGCTCATGCCGGACCCTCCGTAGATTTAACTATCTTCTCTCTTCATTTAGCAGGTGCTTCCTCAATTTTAGGGGCTATTAATTTTATTACTACTGTTATCAA
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Opiliones was found to be negative for Wolbachia. |
Cicurina (Meshweaver spider)
Tufted Clusterfly
Juvenile Pillbug
Dark-winged Fungus Gnat
Elm Seed Beetle