Elm Seed Beetle

July 23, 2026

Sample information
Picture
Entry by: Allison C
Location
Collection date
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
Putative identification Arthropoda Insecta Hemiptera Lygaeidae Arocatus Arocatus melanocephalus

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system 1X Agarose Gel Electrophoresis
Buffer TAE
DNA stain SeeGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
GATCTGGTATAGTAGGATCCTCAATAAGATGAATTATTCGAATTGAATTAGGACAACCGGGAGTATTTATTGGAAACGATCAAATTTATAATGTAATTGTAACAGCCCACGCCTTCATTATAATTTTTTTTATGGTAATACCAATTATAATTGGAGGATTTGGTAACTGACTAGTCCCTTTAATAATCGGAGCACCTGATATAGCTTTTCCACGAATAAATAATATAAGATTCTGACTTTTACCTCCCTCATTAACCTTACTATTATCAAGAAGAATTACTGAAAGAGGAGCAGGAACAGGCTGAACAGTATATCCTCCATTATCAAATAGAATTTTCCATAGAGGAGCTCCTGTAGATATAACTATTTTCTCTTTACATCTAGCAGGAATCTCTTCCATTTTAGGAGCTATTAATTTTATTTCAACAATTATTAATATACGACCTTCAGGAATATCTTCAGAAAAAATTCCTTTATTTGTTTGATCCGTAGGAATTACAGCCTTACTATTATTATTATCATTACCAGTATTAGCCGGAGCTATCACAATATTATTAACAGACCGAAATTTTAATACATCATTCTTCGACCCAACAGGAGGGGGTGATCCTATTTTATATCAACATTTATTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Arocatus melanocephalus was found to be negative for Wolbachia.
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