Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 08/31/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection Date: 08/31/2026 Time: 12:30 Collection Method: Beat Stick and Aerial Stick Location: Located under a “Quercus Virginiana” tree, in the grassy field in front of Lane library Habitat: Large tree, grassy, shaded Specimens collected:2 sets of love bugs and an Ant |
| Putative identification | Arthropoda Insecta |
Methods |
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| Extraction kit | |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
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| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | My confidence level is high because my results reflect the previously collected Diptera insect results, over 80% of previous samples were not infected. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
TAGTTGGAACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCA TCCAGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTATAG TTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGA ATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGG TACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTC TTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATT ACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGC CGGGGCTATTACTATACTATTAACAGATCGAAATTTAAATACNAGTNTCTTTGACCCAGCNNAGGGGGAGACCCTATTTT ATACCNNCATTNATTTTGATTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Insecta was found to be negative for Wolbachia. |
CON-LRM Dermaptera
CON-MEM-Hemiptera
CON-DG Hemiptera
CON-MCH
CON-BJA Hymenoptera