DEMAB Orthoptera

November 2, 2026

Sample information
Picture
Entry by: Alexa B
Location No map location data available.
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Collected -> 12:15pm on 08/31/2026 
  • Tall grass near greenhouse towards the pond
  • (left of Arts drive) 
  • Semi-dry tall grass with thorn plants & deer tracks
  • Collected w/ sweep net
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Gel electrophoresis showed positive for Wolbachia , but no usable sequence came back. Original insect was misplaced, but it was replaced with an insect of the same order.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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