Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | I collected my ant in august on the 31 by the science center and the library |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | my confidence is high |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
AATTGGATCTTNAATAAGAATAATCATTCGCTTAGAATTAGGAACTTG TAACTCATTAATTTTAAATGATCAAATTTATAATTCTATTGTTACTGGTCATGCTTTTATTATAATTTTTTTTATAGTAA TACCATTTATAATTGGAGGTTTTGGAAACTTTCTTGTACCCCTAATACTTGGTGCTCCTGATATAGCCTATCCTCGTATA AATAATATAAGATTTTGATTACTTCCCCCCTCTATTCTTCTATTAACAATCAGAAATTTTATTAATAATGGTGTAGGTAC TGGTTGAACAGTCTACCCTCCCTTAGCTTCCAATATTTATCACAATGGTCCTTCCGTAGATTTAGCCATTTTTTCTCTTC ATATTGCTGGAATATCCTCAATTCTTGGAGCAATTAATTTTATCTCTACTATCTTAAATATACATCATAAAAATTTTTCT ATAGATAAAATTCCTCTCTTAGTTTGAGCAATCTTAATTACTGCAATTCNTCTTTTATTATCACTACCTGTACTTGCCGG ANCTATCNCCATACTTCTAACTGATCGAAATTTANNCNCATCATTTTTTGATCCATCGGGAGGAGGGGACCCNATTTTAT ACCNACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Hymenoptera was found to be negative for Wolbachia. |
CON-LRM Dermaptera
CON-MEM-Hemiptera
CON-DG Hemiptera
CON-MCH
CON-BJA Hymenoptera