Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 08/31/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Yellow Rice Stinkbug with red eyes, six legs, long antennae. |
| Putative identification | Arthropoda Insecta |
Methods |
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| Extraction kit | |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | A DNA extraction kit of in-house reagents was used. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My collected insect was small enough and I properly cut the stinkbug in half as to extract its DNA and Wolbachia DNA. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GTATAGTAGGNTCCGCTATAAGACTAATTATCCGAATCGAACTAGGA CAACCAGGAAGATTCATTGGAGATGATCAAATTTATAACGTAATCGTAACAGCTCACGCCTTCGTAATAATTTTCTTCAT AGTAATACCTATTATAATTGGAGGATTTGGAAACTGACTAGTTCCCCTAATAATCGGAGCTCCCGACATAGCATTCCCTC GTATAAATAACATAAGATTTTGATTATTACCTCCTTCATTAACATTATTAATAATAAGTAGACTAGCAGAATCAGGAGCT GGAACCGGATGAACAGTTTACCCTCCTTTATCAAGTAATTTATCCCACAGAGGAGCATCCGTAGACTTAGCAATTTTTTC ACTCCACTTAGCCGGAGTATCTTCAATCTTGGGAGCAGTAAATTTTATTTCCACAATCATTAATATACGACCCGCAGGAA TAATCCCTGAACGAATTCCTTTATTTGTATGATCAGTAGGGATTACTGCCTTACTTCTACTACTATCATTACCTGTGCTA GCAGGTGCTATTACAATATTATTAACTGATCGAAATTTTAACACATCATTTTTCGATCCCTCAGGTGGAGGAGACCCTAT TTTATACCAACATTTATTTTGATTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Insecta was found to be postive for Wolbachia. |
CON-LRM Dermaptera
CON-MEM-Hemiptera
CON-DG Hemiptera
CON-MCH
CON-BJA Hymenoptera