CON-MEM-Hemiptera

Sample information
Picture	Entry by: Madison M
Location	Approximate location: 31.97, -81.16
Collection date	08/31/2026
Captive / Cultivated?	Wild-caught
Group	Georgia Southern University
Observations	Collection Date ___08/31___ Time of day: ___3:00___ Collection method/equipment: ___Tray and gloves___ Detailed description of the location of the collection site: ___Right outside the science center under the trees in the dirt.___ Detailed description of the habitat of the collection site: ___In the pine straw, dark, moist, small.___
Putative identification	Arthropoda Insecta Hemiptera
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Other
Gel images
Protocol notes	DNA extraction kit of in-house reagents was used.
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	When looking at the GEL electrophoresis system results, my insect shows the band at 406 that represents the Wolbachia strand.
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Hemiptera was found to be postive for Wolbachia.

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