Sample information
Picture	Photos by: Amelia W.
Location
Collection date	02/23/2022
Captive / Cultivated?	Wild-caught
Group	Singapore American School
Observations
Putative identification	Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system
Buffer	PBS
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level	Controls worked.  Did not show Wolbachia nor Arthropod DNA bands in Gel Electrophoresis.
Wolbachia 16S sequence	438 bp BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence	708 bp BLAST at The Wolbachia Project   BLAST at NCBI
Summary	The Drosophila melanogaster was found to be negative for Wolbachia.