Clubiona saxatilis AZ2

May 17, 2022

Sample information
Picture
Photos by: Agata Z.
Location
Collection date 04/05/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Arachnida Araneae Clubionidae Clubiona Clubiona saxatilis

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There is a Cytochromoxydase band and a Wolbachia band
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACATTGGTGGCTACTTTGGGCTGCNANGNNCGAGGCTNAATNTTCCCTTTNAAATCATCAAAAAAAAGAGGGNCTAAACAACACGAGGGGGTGGAAGTGGGANNGCAAGAGAANGTNTNAAATGGAAAGGGGGGGGGGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ANANANAGGGAATGGTNNNNNNTNTNNNANGNGNNNTGGGGNNNNTAACGGNNGNGNGNGGANNTNCTTNNTNNGCGANANNNANNNNTGGNNGATNTCNNCANNCCGGCTGACCNNNNNTTNNNTGNNNNNGGATGNGGCGNNNNNNGNTAGGNGNNTNTTATGNNAGTTTTGANNGGGNGGGNNGATNNTANGATNTTNNNAANNAANAAAAAAAATGGNGTAAAGAAAGNGGGGNGGGGTNTNGNCTAAGAGAANGNNTTNNNTNGAAAGGGGGGGGGGGNANNNTACCNNNNCCGNCNNCNNTNNCCCNCTCCCCCCGCCANGGNCCNNCCCCNTCCNTCAAACCAAACNCTTGCGGNNCCNNNAAACCCGGGGCCCNCTTNNTTNNCATCNTCNNNTCCTANTTAACNTACCTCANNACTNNTCCTNCCTCNTNCCCNCTCTCAAACNACCCACCTTCCCCCNCNNCCCCTCCNCCCCGCCCCCCNCCCCTTNCCNANNNACCNCTTTNTNACCAGAATNCCCCACACANNNCCCCCCCCNCNCCCCCCCACTCCTCACNNCTCCCCCCTNCCNNTCCTCNCNCANCNCNNNNNNCNNCTCNCANNNNCCNNCNGGCCGTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Clubiona saxatilis was found to be postive for Wolbachia.
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