Sample information
Picture	Photos by: Nick J.
Location
Collection date	05/05/2022
Captive / Cultivated?	Wild-caught
Group	Woodford County High School
Observations	Netted Trap, killed with alcohol, hot/humid day, next to a water source
Putative identification	Arthropoda Hexapoda Insecta
Methods
Extraction kit	Instagene Matrix
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	Fast Blast
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Insecta was found to be negative for Wolbachia.