Sample information |
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Picture |
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Location | |
Collection date | 04/30/2021 |
Captive / Cultivated? | Wild-caught |
Group | KantiWil |
Observations | |
Putative identification | Arthropoda Hexapoda Insecta Diptera Trichoceridae Trichocera Trichocera hiemalis |
Methods |
|
Extraction kit | DNeasy (Qiagen) |
DNA extraction location | body without wings |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
|
Protocol notes | |
Results |
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Wolbachia presence | Unknown |
Confidence level | Medium |
Explanation of confidence level | Line 12: no CO1-Band, no W-Band in the same series – Drosophila positive control Vanderbilt worked well, see BK26 |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary |