Sample information
Picture
Location
Collection date	08/30/2022
Captive / Cultivated?	Wild-caught
Group	Antioch High School
Observations	Mottled; speckled; biting; venomous
Putative identification	Arthropoda Arachnida Araneae
Methods
Extraction kit	Wizard SV (Promega)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Edvotek Gel Electrophoresis
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Unknown
Confidence level	Low
Explanation of confidence level	The controls did not work, and the sample data did not show.
Wolbachia 16S sequence
Arthropod COI sequence
Summary