NJ Mosquito- Diya Patel

September 22, 2022

Sample information
Picture
Photos by: Diya P.
Location
Collection date 09/20/2022
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

The male Larva was captured and then cultivated from the Middlesex Observation Commission and then grown into an adult there and fed with human blood and then donated to us at SPHS.
Putative identification Arthropoda Hexapoda Insecta Diptera Culicidae Culex

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Modification P2 for ATL and N3 for AL

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

No band was present in the 500 level which shows that Wolbachia was not present in the mosquito.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Culex was found to be negative for Wolbachia.
Report Inappropriate Post