LS1 Acyrthosiphon pisum

October 26, 2022

Sample information
Picture
Photos by: Lilian S.
Location
Collection date 09/14/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

on gravel bottom
Putative identification Arthropoda Hexapoda Insecta Hemiptera Psyllidae

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Banden im Gel nur schwach erkennbar
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TAACAATAATCAATTATATAATGTAATTGTTACAATTCATGCTTTTATTATAATTTTTTTTATAACTATACCAATTGTAATTGGTGGATTTGGAAATTGATTAATTCCTATAATAATAGGATGTCCTGATATATCATTTCCTCGCTTAAATAATATTAGATTTTGATTATTACCTCCTTCATTAATAATAATAATTTGCAGTTTCTTAATTAATAATGGAACAGGAACAGGATGAACTATTTATCCACCTTTATCAAATAATATTGCACATAATAACATTTCAGTTGATTTAACTATTTTTTCTTTACACCTAGCAGGAATTTCATCAATTTTAGGAGCAATTAATTTTATTTGTACAATTCTTAATATAATACCTAATAACATAAAATTAAATCAAATTCCACTTTTCCCTTGATCAATTTTAATTACAGCTATCTTATTAATTTTATCTTTACCAGTTTTAGCTGGTGCTATTACAATATTATTAACTGATCGAAACTTAAATACATCATTTTTTGATCCAGCAGGAGGAGGAGATCCTATTTTATACCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Psyllidae was found to be negative for Wolbachia.
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