Episyrphus balteatus NL2

October 26, 2022

Sample information
Picture
Photos by: Nikolett L.
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

Found hovering in front of a flower on a field.
Putative identification Arthropoda Hexapoda Insecta Diptera Syrphidae Episyrphus Episyrphus balteatus

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Two bands are clearly visible on the gel.
Wolbachia 16S sequence Download FASTA    Download AB1
GAGTACTTTAAGGAAACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCTTTATGGAGKGGGCTACACACGTGCTACAATGGTGTCTACAATGGGCTGCAAGGYGCGCAAGCTTAAGCTAATCCCTAAWAGACATCTCAGTTCGGATTGTACTCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTAWWWMRAARCTGAGAATATCATCAATTTTAGGAGCTGTAAATTTTATTACAACTGTTATTAATATACGATCTCATGGAATTACTTATGATCGAATACCTTTATTTGTTTGATCAGTTGTAATTACAGCATTATTATTACTTTTATCATTACCTGTATTAGCAGGAGCTATTACTATACTTTTAACTGATCGAAATTTAAATACTTC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TTGAGCTGGATAGTAGGTACATCATTAAGTGTATTAATTCGTGCAGAACTTGGTCATCCTGGTGCTTTAATTGGAGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTGTAATAATTTTTTTTATAGTAATACCTATTATAATTGGAGGATTTGGTAATTGATTAGTTCCATTAATATTAGGAGCTCCTGATATAGCATTTCCTCGTTTAAATAATATAAGTTTTTGACTATTACCTCCTTCTTTAACATTATTATTAGTAAGTAGTATAGTTGAAAATGGAGCTGGAACAGGTTGAACAGTATATCCTCCTCTTTCTGCTGGTATTGCTCATGGAGGAGCTTCTGTAGATTTAGCAATTTTTTCTTTACATTTAGCAGGAATATCATCAATTTTAGGAGCTGTAAATTTTATTACAACTGTTATTAATATACGATCTCATGGAATTACTTATGATCGAATACCTTTATTTGTTTGATCAGTTGTAATTACAGCATTATTATTACTTTTATCATTACCTGTATTAGCAGGAGCTATTACTATACTTTTAACTGATCGAAATTTAAATACTTCATTCTTTGATCCAGCAGGAGGAGGAGATCCAATTTTATATCAACATTTATTTTGATTTTTGGTCACTAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Episyrphus balteatus was found to be postive for Wolbachia.
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