Diamesa nivoriunda

April 26, 2023

Sample information
Picture
Photos by: Sofi M.
Location
Collection date 03/31/2023
Captive / Cultivated? Wild-caught
Group Woodbridge High School
Observations

Black midge. About 1 cm in length.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The controls worked, which was how I knew this arthropod was Wolbachia negative.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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