Small ant

October 2, 2023

Sample information
Picture
Photos by: Thomas C
Location
Collection date 08/25/2023
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations

Small ant on the sidewalk
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I felt confident because I followed all the steps my teacher told me, and all the controls were correct. Also, there were no irregularities in our gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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