Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/29/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | The ant was found in brick ground in August (there were many other ants in the area). The ant is black and the body is very small and is segmented. |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Formicidae was found to be negative for Wolbachia. |
Water Boatman