Portland State University – Acrididae

November 1, 2023

Sample information
Picture
Photos by: Isaac Y.
Location
Collection date 10/09/2023
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Hexapoda Insecta Orthoptera Acrididae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 2 shows a band for COI, Lane 3 is a positive Wolbachia control, and Lane 4 is blank for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Acrididae was found to be negative for Wolbachia.
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