BROACCF23-1 Hemiptera

Sample information

Photos by: Halie B
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
  • 8/21/2023
  • ~1:40 pm
  • Student gardens near Science Center, GSU Armstrong Campus
  • Garden beds and surrounding vegetation were beaten with a stick, insects fell onto a sheet and were collected with forceps
Putative identification Arthropoda Hexapoda Insecta Hemiptera


Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.


Wolbachia presence No
Confidence level High
Explanation of confidence level

Positive, Negative, and No Template Controls were as expected. Sample ID shortened to HRB: Insect CO1 gene was detected, but not Wolbachia 16s gene. Results are conclusive.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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