BROACC-F23-REB Hemiptera

November 6, 2023

Sample information
Picture
Photos by: Rebecca B
Location No map location data available.
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Was caught using sweep net in tall grass next to a fence on Georgia Southern Campus near the green house with overcast weather.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My confidence level is high because the + control, the – control and the NTC all worked as expected.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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