Sample information |
|
Picture |
|
---|---|
Location | |
Collection date | 11/01/2023 |
Captive / Cultivated? | Wild-caught |
Group | John Overton High School |
Observations | I found it hiding under bunches of leaves in around 40-degree weather. It didn’t fly away from me when I tried to scoop it into my container it just walked right into it and allowed me to capture it. |
Putative identification | Arthropoda Hexapoda Insecta Lepidoptera |
Methods |
|
Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
|
Protocol notes | |
Results |
|
Wolbachia presence | |
Confidence level | |
Explanation of confidence level | |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary |