BROACH23TSV Orthoptera

Sample information

Photos by: Thai V.
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
  • Sweep net collection method.
  • 1:30-2:00 P.M. | 8/23/23.
  • Fields on Arts drive at GSU Armstrong campus.
  • Sweep net across tall grass.
  • Pinch bottom of net — >put in jar –> let bugs go in jar.2
Putative identification Arthropoda Hexapoda Insecta Orthoptera


Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.


Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Positive control, negative control, and NTC results are as expected.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be postive for Wolbachia.
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