E5

Sample information

Picture
Location
Collection date 10/17/2022
Captive / Cultivated? Wild-caught
Group College of Eastern Idaho
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Psychodidae

Methods

Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level High
Explanation of confidence level

Clearly had no band, controls worked, and had same appearance as other negatives.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Psychodidae was found to be negative for Wolbachia.
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