Red Click Beetle (Melanotus miscellus)

Sample information
Picture	Photos by: Samvidha M.
Location
Collection date	11/13/2023
Captive / Cultivated?	Wild-caught
Group	Walton High School
Observations	brown/red, oval shaped, two main segments, six legs, small hairs on posterior side
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Elateridae
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Rear half
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain
Gel images
Protocol notes
Results
Wolbachia presence	Unknown
Confidence level	High
Explanation of confidence level	The controls presented the correct bands, and the DNA collection process had no potential errors.
Wolbachia 16S sequence
Arthropod COI sequence	Download FASTA >MELANOTUS MISCELLUS ACATCCCTAAGACTCTTAATTCGAGCAGAATTAGGAAATCCAGGTTCACTTATTGGTAACGATCAAATTT ATAATGTTATTGTTACAGCCCACGCTTTTATTATAATTTTCTTCATAGTTATGCCAATTATAATTGGAGG ATTCGGAAATTGATTAGTTCCTCTGATACTAGGAGCACCAGATATAGCTTTTCCACGAATAAACAACATA AGATTCTGACTTTTACCACCTTCTTTATCACTTTTATTAATAAGAAGAATCGTAGAAAATGGGGCAGGAA CAGGATGAACAGTTTATCCACCTCTTTCATCTAATATTGCCCATAGAGGATCATCTGTAGATCTTGCCAT TTTTAGTCTTCATTTAGCAGGAATTTCGTCTATTCTAGGTGCTGTAAATTTCATTTCAACTGTAATTAAT ATACGATCAACAGGTATTACATTTGACCGAATACCTCTATTCGTTTGAGCTGTAGCTATTACAGCTCTGC TTCTACTCCTTTCTTTACCTGTACTAGCAGGAGCAATTACCATACTTTTAACTGATCGAAATCTAAACAC ATCATTTTTCGACCCTGC BLAST at The Wolbachia Project BLAST at NCBI
Summary

The Wolbachia Project Database