Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 11/13/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Walton High School |
| Observations | brown/red, oval shaped, two main segments, six legs, small hairs on posterior side |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Elateridae |
Methods |
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| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The controls presented the correct bands, and the DNA collection process had no potential errors. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
>MELANOTUS MISCELLUS ACATCCCTAAGACTCTTAATTCGAGCAGAATTAGGAAATCCAGGTTCACTTATTGGTAACGATCAAATTT ATAATGTTATTGTTACAGCCCACGCTTTTATTATAATTTTCTTCATAGTTATGCCAATTATAATTGGAGG ATTCGGAAATTGATTAGTTCCTCTGATACTAGGAGCACCAGATATAGCTTTTCCACGAATAAACAACATA AGATTCTGACTTTTACCACCTTCTTTATCACTTTTATTAATAAGAAGAATCGTAGAAAATGGGGCAGGAA CAGGATGAACAGTTTATCCACCTCTTTCATCTAATATTGCCCATAGAGGATCATCTGTAGATCTTGCCAT TTTTAGTCTTCATTTAGCAGGAATTTCGTCTATTCTAGGTGCTGTAAATTTCATTTCAACTGTAATTAAT ATACGATCAACAGGTATTACATTTGACCGAATACCTCTATTCGTTTGAGCTGTAGCTATTACAGCTCTGC TTCTACTCCTTTCTTTACCTGTACTAGCAGGAGCAATTACCATACTTTTAACTGATCGAAATCTAAACAC ATCATTTTTCGACCCTGC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | |
Water Boatman