Red Click Beetle (Melanotus miscellus)

April 26, 2024

Sample information
Picture
Photos by: Samvidha M.
Location
Collection date 11/13/2023
Captive / Cultivated? Wild-caught
Group Walton High School
Observations

brown/red, oval shaped, two main segments, six legs, small hairs on posterior side
Putative identification Arthropoda Hexapoda Insecta Coleoptera Elateridae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The controls presented the correct bands, and the DNA collection process had no potential errors.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
>MELANOTUS MISCELLUS ACATCCCTAAGACTCTTAATTCGAGCAGAATTAGGAAATCCAGGTTCACTTATTGGTAACGATCAAATTT ATAATGTTATTGTTACAGCCCACGCTTTTATTATAATTTTCTTCATAGTTATGCCAATTATAATTGGAGG ATTCGGAAATTGATTAGTTCCTCTGATACTAGGAGCACCAGATATAGCTTTTCCACGAATAAACAACATA AGATTCTGACTTTTACCACCTTCTTTATCACTTTTATTAATAAGAAGAATCGTAGAAAATGGGGCAGGAA CAGGATGAACAGTTTATCCACCTCTTTCATCTAATATTGCCCATAGAGGATCATCTGTAGATCTTGCCAT TTTTAGTCTTCATTTAGCAGGAATTTCGTCTATTCTAGGTGCTGTAAATTTCATTTCAACTGTAATTAAT ATACGATCAACAGGTATTACATTTGACCGAATACCTCTATTCGTTTGAGCTGTAGCTATTACAGCTCTGC TTCTACTCCTTTCTTTACCTGTACTAGCAGGAGCAATTACCATACTTTTAACTGATCGAAATCTAAACAC ATCATTTTTCGACCCTGC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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