Orthomorpha Coarctata – Aditi

November 17, 2023

Sample information
Picture
Photos by: Aditi S
Location
Collection date 11/14/2023
Captive / Cultivated?
Group Walton High School
Observations

Darker brown with orange sides. Lots of legs and moves fast.
Putative identification Arthropoda

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

I have a medium confidence level because on our results it says that we did not do enough priming so the sample did not test correctly. One of our original controls were contaminated, the water sample, so that could have led to our results not being accurate.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA   
Summary
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